Particle deposition, as Blue Pipette tip, the particles deposited in the cell chamber are all on the glass fiber membrane (Type: MNGF-1; Machery-Nagel, Germany), and the glass fiber membrane passes through the Clip Fit (Figure 4). ) The device is fixed in the cell culture chamber, and the experimental weighing analytical balance (SE2F Microbalance, readability: 0.1 mg; Sartorius GmbH, Germany). Figure 4 Clip Fit Fixing Bracket Figure

5 Particle Deposition Model Figure 3. Results Particles of different sizes and masses have different deposition results in two different exposure modules. Under normal circumstances, although the physical and chemical properties and particle size of the particles may affect the particle deposition, the exposure results of the same particle can be seen that the exposure data of the three positions in the radiation flow exposure system CULTEX RFS has lower exposure data The deviation and relative standard deviation, and the relative standard deviation is within 5%. The relative standard deviations of the linear flow exposure system CULTEX CG are all greater than 10%, and its precision and experimental parallelism and repeatability between the three channels are poor. Table 2 Deposition quality and analysis of Pural 200 on glass fiber membrane after 30 minutes exposure Exposure module Insert 1 [μg] Insert 2 [μg] Insert 3

The particle size of high-purity alumina Pural 200 ranges from 40 nanometers to 40 microns. The linear flow exposure poisoning system and the radiation flow exposure poisoning system use the same aerosol generator. After the same exposure time, the glass fiber membrane was weighed before and after the exposure, and the weight of the particles deposited on the membrane was obtained, as shown in Table 2. 3.1.2

The experimental data of the two exposure modules showed that, on average, the quality of particle deposition was linear Exposure to toxic systems by flow exceeds that of radiation exposure. However, at the same time, the range of changes in the values ​​obtained by the three exposure positions of the radiation flow exposure system was significantly reduced. This means that the three chambers of the radiation exposure system can distribute particles more equally, the parallelism between the channels is better, and the experiment repeatability and stability are better. In order to study this aspect, we further study the other two particulate compounds copper oxide microparticles and copper oxide nanoparticles. 3.2 Copper oxide micron particles The size of copper oxide particles is less than 5mm,

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