Particle deposition, as Blue Pipette tip, the particles deposited in the cell chamber are all on the glass fiber membrane (Type: MNGF-1; Machery-Nagel, Germany), and the glass fiber membrane passes through the Clip Fit (Figure 4). ) The device is fixed in the cell culture chamber, and the experimental weighing analytical balance (SE2F Microbalance, readability: 0.1 mg; Sartorius GmbH, Germany). Figure 4 Clip Fit Fixing Bracket Figure
5 Particle Deposition Model Figure 3. Results Particles of different sizes and masses have different deposition results in two different exposure modules. Under normal circumstances, although the physical and chemical properties and particle size of the particles may affect the particle deposition, the exposure results of the same particle can be seen that the exposure data of the three positions in the radiation flow exposure system CULTEX RFS has lower exposure data The deviation and relative standard deviation, and the relative standard deviation is within 5%. The relative standard deviations of the linear flow exposure system CULTEX CG are all greater than 10%, and its precision and experimental parallelism and repeatability between the three channels are poor. Table 2 Deposition quality and analysis of Pural 200 on glass fiber membrane after 30 minutes exposure Exposure module Insert 1 [μg] Insert 2 [μg] Insert 3
The particle size of high-purity alumina Pural 200 ranges from 40 nanometers to 40 microns. The linear flow exposure poisoning system and the radiation flow exposure poisoning system use the same aerosol generator. After the same exposure time, the glass fiber membrane was weighed before and after the exposure, and the weight of the particles deposited on the membrane was obtained, as shown in Table 2. 3.1.2
The experimental data of the two exposure modules showed that, on average, the quality of particle deposition was linear Exposure to toxic systems by flow exceeds that of radiation exposure. However, at the same time, the range of changes in the values obtained by the three exposure positions of the radiation flow exposure system was significantly reduced. This means that the three chambers of the radiation exposure system can distribute particles more equally, the parallelism between the channels is better, and the experiment repeatability and stability are better. In order to study this aspect, we further study the other two particulate compounds copper oxide microparticles and copper oxide nanoparticles. 3.2 Copper oxide micron particles The size of copper oxide particles is less than 5mm,
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5 Particle Deposition Model Figure 3. Results Particles of different sizes and masses have different deposition results in two different exposure modules. Under normal circumstances, although the physical and chemical properties and particle size of the particles may affect the particle deposition, the exposure results of the same particle can be seen that the exposure data of the three positions in the radiation flow exposure system CULTEX RFS has lower exposure data The deviation and relative standard deviation, and the relative standard deviation is within 5%. The relative standard deviations of the linear flow exposure system CULTEX CG are all greater than 10%, and its precision and experimental parallelism and repeatability between the three channels are poor. Table 2 Deposition quality and analysis of Pural 200 on glass fiber membrane after 30 minutes exposure Exposure module Insert 1 [μg] Insert 2 [μg] Insert 3
The particle size of high-purity alumina Pural 200 ranges from 40 nanometers to 40 microns. The linear flow exposure poisoning system and the radiation flow exposure poisoning system use the same aerosol generator. After the same exposure time, the glass fiber membrane was weighed before and after the exposure, and the weight of the particles deposited on the membrane was obtained, as shown in Table 2. 3.1.2
The experimental data of the two exposure modules showed that, on average, the quality of particle deposition was linear Exposure to toxic systems by flow exceeds that of radiation exposure. However, at the same time, the range of changes in the values obtained by the three exposure positions of the radiation flow exposure system was significantly reduced. This means that the three chambers of the radiation exposure system can distribute particles more equally, the parallelism between the channels is better, and the experiment repeatability and stability are better. In order to study this aspect, we further study the other two particulate compounds copper oxide microparticles and copper oxide nanoparticles. 3.2 Copper oxide micron particles The size of copper oxide particles is less than 5mm,
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The experimental results show that the fear memory time of the VD model group mice is shortened, and the neurons in the hippocampus CA 1 area of the brain are damaged, and the holly moss extract can significantly increase the fear memory time of the VD mice and reduce the neuronal cell damage of the VD mice Combined with its influence on the apoptotic proteins Bcl -2 and Bax, it can be inferred that Mao Dongqing exerts a neuroprotective effect by inhibiting the apoptosis of hippocampal CA1 area.1 Pole-climbing experiment on the 30th day 2h after the administration, the pole-climbing experiment was tested. The scene is the same as the scene in the training stage.
10 Statistical processing method SPSS 17. 1 Materials and methods 1.09, indicating that the VD model was successfully replicated and the memory ability of the mice was reduced. The hippocampus is related to brain memory and is sensitive to Cio7 for cerebral ischemia. by
Department of Centrifuge tube Rack Manufacturers Identification, Guangzhou University of Chinese Medicine. After the perfusion was completed, the brain tissue was taken and fixed in 10% neutral formalin solution for pathological section preparation. The rod climbing experiment was used to evaluate the differences in the coordination ability of mice between each group. A total of 4 cycles with an interval of 15 s each.2 The effect of PHRE on the conditioned fear memory of mice.5 cm was fixed on the foam box, and the bamboo pole was wrapped with gauze to prevent slipping Place the mouse head down on the top of the bamboo pole, and record the time it takes for the mouse to climb the full length of the pole.3 Instrument AUM 220D electronic analytical balance, Japan Shin ad zu company ;Ultra-low temperature refrigerator, American Thermo company;
Ice maker, Japanese Sanyo company product; High-speed refrigerated centrifuge, China Zhongke Zhongjia Science and Technology Instrument Co. Impairment, memory impairment; Compared with the model group, Ilex glabra extract can increase the expression of Bcl -2 and reduce the expression of B ax, thereby significantly increasing the ratio of Bc}2 to ax and causing the apoptosis of hippocampal CA1 cells inhibition. Keywords: Ilex extract; Vascular dementia; Learning and memory ability
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10 Statistical processing method SPSS 17. 1 Materials and methods 1.09, indicating that the VD model was successfully replicated and the memory ability of the mice was reduced. The hippocampus is related to brain memory and is sensitive to Cio7 for cerebral ischemia. by
Department of Centrifuge tube Rack Manufacturers Identification, Guangzhou University of Chinese Medicine. After the perfusion was completed, the brain tissue was taken and fixed in 10% neutral formalin solution for pathological section preparation. The rod climbing experiment was used to evaluate the differences in the coordination ability of mice between each group. A total of 4 cycles with an interval of 15 s each.2 The effect of PHRE on the conditioned fear memory of mice.5 cm was fixed on the foam box, and the bamboo pole was wrapped with gauze to prevent slipping Place the mouse head down on the top of the bamboo pole, and record the time it takes for the mouse to climb the full length of the pole.3 Instrument AUM 220D electronic analytical balance, Japan Shin ad zu company ;Ultra-low temperature refrigerator, American Thermo company;
Ice maker, Japanese Sanyo company product; High-speed refrigerated centrifuge, China Zhongke Zhongjia Science and Technology Instrument Co. Impairment, memory impairment; Compared with the model group, Ilex glabra extract can increase the expression of Bcl -2 and reduce the expression of B ax, thereby significantly increasing the ratio of Bc}2 to ax and causing the apoptosis of hippocampal CA1 cells inhibition. Keywords: Ilex extract; Vascular dementia; Learning and memory ability
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The unbalance of the rotor will cause the motor shaft to swing, affecting the life of the centrifuge. In severe cases, the motor shaft will be broken or even exploded. head. Generally, the unbalance is detected by detecting the swing of the motor shaft. ④Improving the centrifuge’s ability to resist imbalances not only improves the robustness of the centrifuge,
but also does not require strict balance during the operation of the rotor, and even visual balance can be used, which is convenient for users. In general, small centrifuges place the entire motor on a shock-resistant elastic support, such as rubber pads, etc., and high-speed centrifuges often use a flexible shaft to resist shock. ⑤The rotor identification system can automatically identify the rotor, limit the maximum speed of the rotor, and effectively prevent the user’s misoperation.
The basic principle of rotor identification is to set a unique code for each rotor, or inlay a certain number of small magnets at the bottom of each rotor, use sensing, or use a photoelectric refraction ring sensor, more complex ultrasonic and The identification of the wireless RF rotor, the rotor can be identified without running, and even the rotor cover can be detected. Environmental protection is mainly manifested in two aspects. Noise reduction. Generally speaking, the talking sound is usually 60 points p, and the sound of public gas cars is 70 to 80 decibels. Medical research shows that noise above 70 decibels can interfere with the conversation, causing upset and affecting work. ,
Or even cause an accident, according to the technical evaluation specification for measurement certification (fiG102-90) of China’s product quality inspection agency, the noise of the test laboratory should not be greater than 70dBo. The noise reduction measures of the centrifuge usually are: shell and rotating head cavity plus sound-absorbing coating Layer; adopt strong and stable anti-seismic base ton machine and mechanical frame with elastic shock absorber isolation; apply motor without commutator, etc. Generally, after the shock absorption treatment, the noise of the centrifuge can be limited to 60 decibels.
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but also does not require strict balance during the operation of the rotor, and even visual balance can be used, which is convenient for users. In general, small centrifuges place the entire motor on a shock-resistant elastic support, such as rubber pads, etc., and high-speed centrifuges often use a flexible shaft to resist shock. ⑤The rotor identification system can automatically identify the rotor, limit the maximum speed of the rotor, and effectively prevent the user’s misoperation.
The basic principle of rotor identification is to set a unique code for each rotor, or inlay a certain number of small magnets at the bottom of each rotor, use sensing, or use a photoelectric refraction ring sensor, more complex ultrasonic and The identification of the wireless RF rotor, the rotor can be identified without running, and even the rotor cover can be detected. Environmental protection is mainly manifested in two aspects. Noise reduction. Generally speaking, the talking sound is usually 60 points p, and the sound of public gas cars is 70 to 80 decibels. Medical research shows that noise above 70 decibels can interfere with the conversation, causing upset and affecting work. ,
Or even cause an accident, according to the technical evaluation specification for measurement certification (fiG102-90) of China’s product quality inspection agency, the noise of the test laboratory should not be greater than 70dBo. The noise reduction measures of the centrifuge usually are: shell and rotating head cavity plus sound-absorbing coating Layer; adopt strong and stable anti-seismic base ton machine and mechanical frame with elastic shock absorber isolation; apply motor without commutator, etc. Generally, after the shock absorption treatment, the noise of the centrifuge can be limited to 60 decibels.
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